The effect of polymer backbone chemistry on the induction. Radically modified cells may stunt brain tumor growth 4 february 2014, by bobbie mixon brain cancers hide behind a protective barrier in the brain and attack white blood cells. The existence of polymer layers on the liposome surface was confirmed by measuring the zeta potential of the liposomal particles. Sep 19, 2012 liposomes are ubiquitous components of skin moisturizers and other personalcare products. Caged nanobins as a theranostic platform with enhanced magnetic resonance relaxivity and ph. Furthermore, the use of synthetic polymers enables designers to manipulate the characteristics of the membrane and thus control permeability, release rates, stability and other properties of the polymersome. Characterization and release kinetics of liposomes. Request pdf proteasesensitive, polymercaged liposomes. These modified liposomes possess surfaceactive carboxylate groups that can be crosslinked with telechelic 2,2ethylenedioxybisethylamine linkers, resulting in polymer caged liposomes pcls that are highly stable and have tunable phsensitive responses.
When this system is used for drug delivery, both hydrophilic and lipophilic drugs can be transported therein torchilin, 2007. Today, they are a very useful reproduction, reagent, and tool in various scientific disciplines, including mathematics and theoretical physics, biophysics, chemistry, colloid science, biochemistry, and biology. Tunable surface properties of temperatureresponsive polymer modified liposomes induce faster cellular uptake, acs omega 2017. A method for making highly targeted liposomes using triggered release. Recent trends of polymer mediated liposomal gene delivery. Linear polyllysine, protamine, histone, and various synthetic polypeptides have been used as the dna. Ijms free fulltext preparation and characterization of. A scheme showing the multiple steps involved in the synthesis of upasensitive, polymer caged liposomes. The hct116 cells were washed twice with pbs solution, and fresh ph 7. Ijms free fulltext preparation and characterization.
The diacid metabolite of norcantharidin dmnctd is clinically effective against hepatocellular carcinoma hcc, but is limited by its short halflife and high incidence of adverse effects at high doses. Advanced and targeted drug delivery market to 2021. Introduction liposomes are simple microscopic vesicles in which an aqueous volume is entirely enclosed by a membrane composed of a lipid molecule. Preparation and characterization of electrostatically. These liposomes are composed of natural phospholipids which may be neutral or negatively charged and cholesterol. One common method to improve liposomal stability is to surface modify the liposomes by coating with a polymer takeuchi et al. The detail about lipid polymer hybrid nanoparticles lphnps is described in this presentation. Today, they are a very useful reproduction, reagent, and tool in various. A method for making highly targeted liposomes using triggered release liposomes. Liposomes have been used extensively for numerous applications, including as drug delivery systems. These liposomes are often used for targeting of the reticuloendothelial system res. A phresponsive delivery system with high stability polymerincorporated liposomes were prepared from preformed liposomes and a cholesterolfunctionalized. The crosslinked polymer cage in pcn dxr offers protection for the drug payload while serving as a phresponsive trigger that enhances drug release in the acidic environments commonly seen in. Polymers in reservoir based drug delivery systems have shown immense progress in the form of hydrogels and liposomes.
Most reported polymersomes contain an aqueous solution in their core and are useful for encapsulating and. Liposomes were synthesized that had a diameter of 6 nm. Characterization of liposomes the liposomes prepared were characterized by their phospholipid contents by. Pdf folate receptortargeted liposomes loaded with a diacid. Request pdf proteasesensitive, polymer caged liposomes. A method for making highly targeted liposomes using triggered release mt basel, t shrestha, dl troyer, sh bossmann acs nano 5. Among nonviral vectors, cationic liposomes and cationic polymers have been more commonly used 11, because they can easily form complexes with the dna or rna molecules via the negatively charged phosphate groups of nucleic acids. B proposed mechanism of action of the polymer caged liposomes in response to presence of enzyme. Dec 03, 2007 the resulting polymer caged liposomes are stable, both in serum and during freezedrying and rehydration. Compositions and methods for polymercaged liposomes patent.
These modified liposomes possess surfaceactive carboxylate groups that can be crosslinked with telechelic 2,2ethylenedioxybisethylamine linkers, resulting in polymercaged liposomes pcls that are highly stable and have tunable phsensitive responses. This latter observation is a promising observation for future commercial production and shelflife concerns. Synthesis and characterization of ni ii, as iiiencapsulated polymercaged nanobin, pcnni,as ni ii, as iiiencapsulated bare liposomes, blni,as, were first prepared from aqueous ato and nickel acetate nich 3 coo 2 using a previously reported igm drugloading protocol. Since then, liposomes have made their way to the market. Zwitterionic polycarboxybetainebased cationic liposomes. Utilization of chitosan caged liposomes to push the boundaries of therapeutic delivery. Liposomes, sphereshaped vesicles consisting of one or more phospholipid bilayers, were first described in the mid60s. Recent trends of polymer mediated liposomal gene delivery system.
Polymeraugmented liposomes enhancing antibiotic delivery against intracellular infections fangyi su, a jasmin chen, a hyenam son, a abby m. The mucoadhesive function of the polymer coated liposomes was evaluated in vitro using rat intestine. With nearly one hundred years of intensive study, lipids have proven to be a vital and evermorepromising area of cell biological research. Feb 22, 20 research on liposome technology has progressed from conventional vesicles to secondgeneration liposomes, in which longcirculating liposomes are obtained by modulating the lipid composition, size, and charge of the vesicle.
Liposomes with modified surfaces have also been developed using several molecules, such as glycolipids or sialic acid. Polymercaged liposomes when polymers are anchored on the surface of liposomes, polymercaged liposomes are formed which are more stable systems. Utilization of chitosancaged liposomes to push the. Liposomes can be coated with a functionalized polymer, creating a nanobin, to improve targeted drug delivery. Lipidpolymer hybrid nanocarriers for delivering cancer. Progress in the development of lipoplex and polyplex modified. Contents of these liposomes are most often destined for lysosomes new, 1990. Seminar on characterization and stability of liposomes. The present invention provides liposomal compositions and methods of using such compositions in vitro and in vivo. In such case aquasomes proof to be worthy carrier, which are comprised of solid carriers whose film has been treated with a. Biological evaluation of phresponsive polymercaged.
Upon addition of the polymer the diameter increased by 2. Handling of liposomes the lipids used in the preparation of liposomes are unsaturated and hence susceptible to oxidation. Liposomes modified with temperatureresponsive polymers. Polymer cage stabilizes liposome, improves drug delivery. The drugs are often inevitable and these always bring limitation to drug delivery system. The reported lipidpolymer hybrid systems include dna precondensed with polycations followed by coating with cationic liposomes 145, 146, anionic liposomes, or amphiphilic polymers with or without helper lipids. Pdf folate receptortargeted liposomes loaded with a. The mucoadhesive function of the polymercoated liposomes was evaluated in vitro using rat intestine. Structurally, liposomes are concentric bilayered vesicles in which an aqueous volume is entirely enclosed by a membranous lipid bilayer mainly composed of natural or synthetic phospholipids. Tunable surface properties of temperatureresponsive polymermodified liposomes induce faster cellular uptake, acs omega 2017.
The reported lipid polymer hybrid systems include dna precondensed with polycations followed by coating with cationic liposomes 145, 146, anionic liposomes, or amphiphilic polymers with or without helper lipids. Progress in the development of lipoplex and polyplex. However, the polymercaged liposomes exhibited slow drug release profiles. The combination of liposomes with polymeric scaffolds could revolutionize the current state of drug delivery technology. A scheme showing the multiple steps involved in the synthesis of upasensitive, polymercaged liposomes. Tougher liposomes although liposomes can be used for drug delivery, efforts continue toward improving both the stability of these spherical bilayer structures and the control over how they release their aqueous contents. B proposed mechanism of action of the polymercaged liposomes in response to presence of enzyme. Two approaches for overcoming the factors related to the suboptimal efficacy of. Doxloaded ecmtargeting liposomes, doxloaded polymerincorporated liposomes, and free dox in ph 7.
Liposomes are ubiquitous components of skin moisturizers and other personalcare products. After crosslinking with either a short peptide containing a lysine so that it is a diamine or ethylenediamine, the diameter increased between 5. Nov 14, 2007 proteasesensitive, polymer caged liposomes. Cationic polymertherapeutic gene complexes cationic polyplexes or cationic. A method for making highly targeted liposomes using triggered release mt basel, t shrestha, dl troyer, sh bossmann acs nano 5 3, 21622175, 2011. A method for making highly targeted liposomes using triggered release liposomes have become useful and wellknown drug delivery. Advanced and targeted drug delivery market to 2021 nanoparticles, polymers, liposomes, micelles, nanoemulsions, dendrimers, monoclonal antibodies by geography, therapeutic area and stakeholder. Methods and protocols promises to be an essential source of practical knowhow for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol. In biotechnology, polymersomes are a class of artificial vesicles, tiny hollow spheres that enclose a solution. Stimuliresponsive materials as intelligent drug delivery.
Tremendous use of polymers has been witnessed in the area of polymer therapeutics and nano medicines 1. Less than 20% of the payloads were released within 24 h owing to the covalent bonds that irreversibly occupied the functional groups that were essential for ph sensitivity. In contrast, freezedrying and rehydration destroy conventional liposomes. Liposomes modified with temperatureresponsive polymers are. Two approaches for overcoming the factors related to the. This shortens the circulation times of the liposomes substantially.
In order to ensure that liposomes are suspended in the polymer matrix the capacity of the polymer to induce the liposome aggregation should be determine. However, liposomes are often prone to stability problems. The resulting polymercaged liposomes are stable, both in serum and during freezedrying and rehydration. Reprinted with permission from reference 15, american chemical society 2011. Enzymeequipped liposomes embedded in polymer capsules as a novel biomedical transport system when cells cannot carry out the tasks required of them by our. In this study, we utilized electrostatic interactions as the. Linear polyllysine, protamine, histone, and various synthetic polypeptides have been used as the dna condensation component. May 24, 2011 synthesis and characterization of ni ii, as iiiencapsulated polymer caged nanobin, pcnni,as ni ii, as iiiencapsulated bare liposomes, blni,as, were first prepared from aqueous ato and nickel acetate nich 3 coo 2 using a previously reported igm drugloading protocol. A particle counting method using the coulter counter was adopted to evaluate the adhesive % of liposomes.
Triggered release of pharmacophores from nihaso3loaded. According to the weak acid of tumor extracellular environment, a phsensitive bolatype triblock copolymer peg m pdpa n peg m was synthesized and mixed with phospholipid to form functional hybrid liposomes. In particular, the present invention provides stable, polymer caged liposomes comprising a ph responsive delivery mechanism for delivery of nucleic acids, peptides, small molecules, drugs, vitro and in vivo. Characterization and release kinetics of liposomes inserted. In vitro studies have demonstrated that charged liposomes adsorb to hydroxyapatite ha, a model substance for human dental enamel nguyen et al.
The particle size of the crosslinked polymerliposomes was approximately 140 nm and the polymerliposomes were loaded with the anticancer drug doxorubicin. A phresponsive delivery system with high stability polymer incorporated liposomes were prepared from preformed liposomes and a cholesterolfunctionalized. Lipid polymer hybrid nanoparticles liposome nanoparticle. Preparation, characterization and applications of liposomes. Zwitterionic polymer based lipid distearoyl phosphoethanolaminepolycarboxybetaine 20 dspepcb 20 was used to make modification of cationic liposomes as it had comparable ability in enhancing the serum stability of cationic liposomes with that of dspepeg 2000 in our previous work figure 1a. Radically modified cells may stunt brain tumor growth. Biological and pharmaceutical evaluation of the elaborated colloidal dispersion systems in terms of interaction with the mucosal membranes. One important thing in tumor chemotherapy is to develop the targeting, precise timing, and quantitative drug deliveryrelease system. Matt basel faculty anatomy and physiology kansas state. Although liposomes have been extensively studied as a promising drug delivery model for bioactive compounds, there still remain major drawbacks for widespread pharmaceutical application. Methods and protocols, leading experts in the related fields explore cuttingedge experimental methods involving all aspects of lipids as essential components of the cell membrane.
In the presence of nucleic acidmodified liposomes, complementary to the uncaged, hairpinfragmented modified liposomes, interliposome, duplexcrosslinked, structures are. Author links open overlay panel sonia alavi a azadeh haeri a simin dadashzadeh a b. We developed a dmnctdloaded, folic acid famodified, polyethylene glycolated dmnctdfapeg liposome system to enhance the targeting effect and antitumor potency for. Polymeraugmented liposomes enhancing antibiotic delivery. Also volatile solvents such as chloroform which are used will tend to evaporate from the container. Apr 14, 2016 one important thing in tumor chemotherapy is to develop the targeting, precise timing, and quantitative drug deliveryrelease system. The effect of polymer backbone chemistry on the induction of. A series of doxorubicinloaded polymercaged nanobins pcn dxr were evaluated in vivo in a murine mdamb231 xenograft model of triplenegative breast cancer. A phresponsive delivery system with high stability sangmin lee, haimei chen, christine m. Cationic polymer therapeutic gene complexes cationic polyplexes or cationic. Since peg is a flexible, watersoluble polymer, it can be used to create very high osmotic pressures on the order of tens of atmospheres.
The proposed bioadhesion of pectin to mineral surfaces the teeth can be based on a similar mechanism as mucosal adhesion hagesaether and sande, 2007, joergensen et al. Polyethylene glycol has a low toxicity and is used in a variety of products. The polymer is used as a lubricating coating for various surfaces in aqueous and nonaqueous environments. We developed a dmnctdloaded, folic acid famodified, polyethylene glycolated dmnctdfapeg liposome system to enhance the targeting effect and antitumor potency for hcc at a moderate. Min lee department of chemistry and the center of cancer nanotechnology excellence, northwestern university, 2145 sheridan rd. Modified liposomes prepared from receptorlike molecules open up fresh opportunities for therapeutic and. While having many of the properties of natural liposomes, polymersomes exhibit increased stability and reduced permeability. In particular, the present invention provides stable, polymercaged liposomes comprising a ph responsive delivery mechanism for delivery of nucleic acids, peptides, small molecules, drugs, vitro and in vivo.
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